chondrocyte differentiation medium  (Cell Applications Inc)

Journal: Cells

Article Title: Heat-Treated Lacticaseibacillus rhamnosus Strains Modulate Inflammatory and Metabolic Processes in In Vitro Systems Relevant to Canine Osteoarthritis

doi: 10.3390/cells15040336

Figure Lengend Snippet: Screening of the anti-inflammatory effect of different heat-treated strains in canine chondrocytes. IL-6 secretion by primary canine chondrocytes stimulated with IL-1β was measured in the presence of a collection of candidates. Data are represented as the percentage of IL-6 secretion relative to IL-1β stimulated condition without inactivated cells. Control condition refers to chondrocytes stimulated with IL-1β, whereas negative control (NC) corresponds to chondrocytes without stimulation. Data are presented as the mean ± SD of three independent experiments. Statistical test: One-way ANOVA with Dunnett’s multiple comparisons test (Postbiotic vs. Control). Significance levels were indicated as follows: p -value > 0.05 (ns: non-significant), p -value < 0.01(**), p -value < 0.0001 (****).

Article Snippet: Alginate beads were washed 5 times with NaCl solution (0.9%) and finally, one more time in chondrocyte differentiation medium (Cell Applications Inc., San Diego, CA, USA).

Techniques: Control, Negative Control

Journal: Cells

Article Title: Heat-Treated Lacticaseibacillus rhamnosus Strains Modulate Inflammatory and Metabolic Processes in In Vitro Systems Relevant to Canine Osteoarthritis

doi: 10.3390/cells15040336

Figure Lengend Snippet: Insoluble type II collagen production by primary canine chondrocytes cultured in spheroids structure. Data are presented as the mean ± SD of three independent experiments and expressed as the percentage of insoluble type II collagen production relative to the control condition. In the control condition, canine chondrocytes were cultured without supplementation of heat-treated strains. Statistical test: Kruskal-Wallis test, followed by Dunn’s multiple comparison test (Control vs. Postbiotic). Significance levels were indicated as follows: p -value < 0.05 (*).

Article Snippet: Alginate beads were washed 5 times with NaCl solution (0.9%) and finally, one more time in chondrocyte differentiation medium (Cell Applications Inc., San Diego, CA, USA).

Techniques: Cell Culture, Control, Comparison

Journal: Regenerative Therapy

Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

doi: 10.1016/j.reth.2025.05.008

Figure Lengend Snippet: Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Article Snippet: The cells were then cultured in canine chondrocyte differentiation medium (Cell Applications) for 30 days at 37 °C and 5 % CO 2 .

Techniques: Derivative Assay, Staining, Quantitative Proteomics, Standard Deviation, Marker